Distribution of endocrine-disrupting PCBs in hard roes of loaches and their potential ecological effects in Weishanhu Lake, China

Preliminary study is carded out on the endocrine-disrupting organic chemicals of polychlorinated biphenyls( PCBs. PCB-28, 60,104, 153, 190) in hard roes of Ioaches( Misgurnus anguillicaudatas) in Weishanhu Lake, China. Results showed that the hard roes of Ioacbes collected from the areas near the influx( such as Pengkou T-PCB =0. 243μg/g) or efflux( such as Hanzhuang T-PCB = 0. 221μg/g)are characterized by higher PCB contents, about 4 times that from the central lake(T-PCB = 0.066-μg/g), suggesting great difference between their pollutions. The PCB distributions indicated that PCB congeners with more chlorine are more easily accumulated in roes of Ioachee. The microscopic characteristics reveal that the PCB contents at present cannot lead the roes of Ioaches to be abnormal.However, the roes with higher PCBs in Pengkou and Hanzhuang are obviously bigger than those with lower PCBs(T-PCBs≤0. 176μg/g)in other localities. It is suggested that PCBs have a stronger estrogenic activity on the roes of Ioaches, and the phenomenon is likely premonitory for the abnormal development of the hard roes.     

加速溶剂萃取-气相色谱-质谱法同时测定土壤中7种Aroclor系列多氯联苯

采用加速溶剂萃取,Florisil固相萃取小柱净化,GC-MS法同时测定土壤中7种多氯联苯(Aroclor 1016、1221、1232、1242、1248、1254、1260)的含量。建立了一种全新的Aroclor系列多氯联苯的定量测定方法,根据氯代联苯峰在单种Aroclor和7种Aroclor混合物中峰高比值(K)固定不变的原理,通过7种Aroclor混合物的标准曲线,计算得出各种Aroclor的标准曲线,测定样品中Aroclor的含量;采用建立多元一次方程组的方法,测定样品中多种不同浓度Aroclor的含量,大大提高了分析效率。7种Aroclor混合物和Aroclor1242、Aroclor1260的土壤加标样品回收率为90.5%~109%,RSD为0.96%~9.38%(n=5),均能满足分析准确度和精密度的要求。